Identification Of Bacteria In Seaweed (Kappaphycus alvaezii) Infected With Ice-Ice Disease In Tarakan City: Bahasa Indonesia

Burhanuddin Ihsan; Kartina Kartina; Rully Fadliansyah

doi:10.32663/ja.v20i2.3110

Authors

Burhanuddin Ihsan Program Studi Akuakultur Fakultas Perikanan dan Ilmu Kelautan Universitas Borneo Tarakan http://orcid.org/0000-0002-1184-8096
Kartina Kartina Program Studi Akuakultur Fakultas Perikanan dan Ilmu Kelautan Universitas Borneo Tarakan
Rully Fadliansyah Program Studi Akuakultur Fakultas Perikanan dan Ilmu Kelautan Universitas Borneo Tarakan

DOI:

https://doi.org/10.32663/ja.v20i2.3110

Keywords:

bacteria, infection, isolation, pathogen, fishery

Abstract

Seaweed (Kappaphycus alvarezii) is a fishery product that has enormous potential as well as has high economic value with very diverse processed products that can improve the welfare of coastal communities. However, the problem that often arises in seaweed cultivation is the presence of ice-ice disease. Symptoms of ice-ice disease are generally characterized by bleaching at the base of the thallus, middle and tip, which begins with a change in color of the thallus to clear white or transparent. This study aimed to analyze the pathogenic bacteria in seaweed (K. alvarezii) which was attacked by ice-ice disease in Tarakan City. Bacterial isolation was carried out by smoothing 30 grams of seaweed samples that had been taken and then 5 grams of it put into an Erlenmeyer containing 250 ml of APW enrichment media and then incubated for 6-8 hours at 37⁰C. Furthermore, the sterilized spherical needle was inserted into the APW medium and then streaked on TCBS and 3% TSA media using the streak plate method and incubated at 37⁰C for 24 hours. Then the bacterial colonies that grew on the media were selected with different colors, shapes and elevations to be purified. Identification of bacteria using the Manual for the Identification of Medical Bacteria and Bergey's Manual of Determinative Bacteriology based on morphological and biochemical characteristics. The results of the study by identifying bacteria in seaweed that were attacked by ice-ice disease based on morphological and biochemical tests found the infecting bacteria were Actinobacillus, Moraxella, Alcaligenes, Vibrio and Bacillus. In conclusion, there were 5 pathogenic bacteria identified in seaweed that was attacked by ice-ice disease including; Actinobacillus, Moraxella, Alcaligenes, Vibrio and Bacillus

References

Cowan & Steel’s. (1974). Manual For the Identification of Medical Bacteria. (Second Edition). London. Cambridge University Press.
[DKP] Dinas Kelautan dan Perikanan. (2019). Data Produksi Perikanan Provinsi Kalimantan Utara. Bulungan.
Erbabley., Nally. Y.G.F., & Kelabora. D.M. (2018). Identifikasi bakteri rumput laut Kappaphycus alvarezii berdasarkan musim tanam di perairan Maluku Tenggara. Jurnal Akuatika Indonesia, 3 (1),19-25. DOI:10.24198/jaki.v3i1.23398.
Holt, JG., Krieg NR, Sneath PHA, Staley JT, & Williams ST. (1994). Bergey’s Manual of Determinative Bacteriology. Ninth Edition. Sans Taghe.
Ihsan, B. (2021). Identifikasi bakteri patogen (Vibrio spp. dan Salmonella spp.) yang mengontaminasi ikan layang dan bandeng di pasar tradisional. Jurnal Pengolahan Hasil Perikanan Indonesia, 24(1),89-96. https://doi.org/10.17844/jphpi.v24i1.34198.
Ihsan. B. (2021). Dasar-Dasar Mikrobiologi. Insan Cendikia Mandiri. Solok. ISBN: 978-623-348-191-5. 159 hal.
Ihsan, B., & Retnaningrum, E. (2017). Isolasi dan identifikasi bakteri Vibrio sp. pada kerang Kapah (Meretrix meretrix) di Kabupaten Trenggalek. Jurnal Harpodon Borneo, 10(1),23-27. DOI: https://doi.org/10.35334/harpodon.v10i1.196.
Ihsan, B., & Retnaningrum, E. (2020). The numerical phenetic of taxonomy Vibrio in Shellfish (Meretrix meretrix) at edu-tourism mangrove Cengkrong Beach Trenggalek. Jurnal Ilmiah Perikanan dan Kelautan, 12(2),296-301.http://doi.org/10.20473/jipk.v12i2.17846.
Maria, L.S., Orbita, J.A., & Arnaiz. (2014). Seasonal changes in growth rate and carrageenan yield of Kappaphycus alvarezii and Kappaphycus striatum (Rhodophyta, Gigartinales) cultivated in Kolambugan, Lanao del Norte. Advances in Agriculture & Botanics-International Journal of the Bioflux Society, 6(2).doi:10.1007/s10811-008-9360-2.
Maryunus. RP. (2018). Pengendalian penyakit ice-ice budidaya rumput laut, Kappaphycus alvarezii: korelasi musim dan manipulasi terbatas lingkungan. Jurnal Kebijakan Perikanan Indonesia, 10(1),1-10.DOI: http://dx.doi.org/10.15578/jkpi.10.1.2018.1-10.
Rahayu SA, Gumilar MH. (2017). Uji cemaran air minum masyarakat sekitar Margahayu Raya Bandung dengan identifikasi bakteri Escherichia coli. Indonesian Journal of Pharmaceutical Science and Technology, 4(2),50-56.DOI:10.15416/ijpst.v4i2.13112.
Strickland, J.D.H. & Parsons, T.R. (1968) A Practical Handbook of Seawater Analysis. Bulletin of Fisheries Research Board of Canada, 167, 1-311.
Syamsuliah. (2021). Identifikasi bakteri pada rumput laut Kappaphycus alvarezii Yang Terinfeksi Penyakit Ice-Ice. Prosiding Seminar Nasional. Politeknik Pankep. Hal 268-274. https://ojs.polipangkep.ac.id/index.php/proppnp/article/view/145.
Vairappan, C.S. (2006). Seasonal occurrences of epiphytic algae on the commercially cultivated red alga Kappaphycus alvarezii (Solieriaceae, Gigartinales, Rhodophyta), J. Appl. Phycol. 18, 611–617. https://doi.org/10.1007/s10811-006-9062-6.
Wulandari SA, Isdiantoni, Prasetyo EN. (2020). Analisis fisika-kimia perairan dan komunitas bakteri terkait kemunculan penyakit ice-ice pada rumput laut (Kappaphycus alvarezii). Science Education and Application Journal, 2(2),66-78. DOI: https://doi.org/10.30736/seaj.v2i2.277