Variation in yield components and bioactive contents of black cumin seeds (Nigella sativa L.) with application of animal manures in ultisol lowland of Bengkulu

  • Herlina Herlina Universitas Dehasen Bengkulu
  • Evi Andriani Agribusiness Study Program, Faculty of Agriculture, University of Dehasen Bengkulu


Black cumin (Nigella sativa L) is known as herbal medicine that grows in subtropical regions and spreads from Asia to Europe. Plant parts that are used are seeds that contain bioactive compounds such as thymoquinone and thymol.  Information about the growth and production from Indonesia is still limited. Therefore it is necessary to develop efforts to cultivate black cumin in Indonesia, especially in sub-optimal land as initial information in the field of plant adaptation studies. This study aimed to determine the effect of manure on yield components and bioactive content of black cumin in the ultisol lowland of Bengkulu. The study was conducted at Dehasen Bengkulu University research station at an altitude below 100 m above sea level. The accessions of India, Syria, and Kuwait were used at the main plot, whereas media for growing viz cow manure, chicken manure, and without manure as a subplot. The study was conducted in February 2019 using the Split Plot Design with three replications. Observations were made on the components of yields and bioactive levels of seeds. The results showed that the application of chicken manure increased the components of the yield of black cumin seed by parameter number of capsules per plant, number of seeds per capsule, and number of seeds per plant and tended to reduce the weight of 1000 seeds. An increasing level of thymoquinone in the cultivation of black cumin in ultisol lowland of Bengkulu was obtained in the cow manure application. The increased levels of seed thymoquinone were accompanied by decreased levels of thymol for all accession used.


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Abas-Ali, M., Sayeed M.A., Shahinur-Alam, M., Yeasmin, M.S., Khan, A.M., Muhamad, I.I. (2012). Charateristics of oils and nutrients contents of Nigella sativa L. and Trigonella foenum-graecum seeds. Buletin of the Chemical Society Ethiopia. 26(1), 55-64.
Abdolrahimi, B., Mehdikhani, P., Hasanzadeh-Gorttappe, A. (2012). The effect of harvest index, yield and yield components of three varieties of black seed (Nigella sativa L.) in different planting densities. Int. J. Agriscience. 2(1), 93-101.
Adimihardja, A., Juarsah, I., Kurnia., U. (2000). Pengaruh penggunaan berbagai jenis dan takaran pupuk kandang terhadap poduktivitas tanah Ultisols terdegradasi di Desa Batin, Jambi. Halaman 303-319 dalam Prosiding Seminar Nasional Sumber Daya Tanah, Iklim, dan Pupuk Buku II, Bogor.
Ahmad, A., Diwan, H., Abrol, Y.P. (2010). Global climate change, stress and plant productivity. In Pareek A, Sopony SK, Bohnert HJ (eds.), Abiotic stress adaption in plants. Springer Link. Pp. 503-521
Ahmadian A., Tavassoli A. and Amiri E. (2011): The interaction effect of water stress and manure on yield components, essential oil and chemical compositions of cumin (Cuminum cyminum). African J. of Agricultural research. 6(10), 2309-2315.
Al-Saleh, I.A., Billedo, G., and El-Doush, I.I. (2006) Level of Selenium, Tocopherol, Thymoquinone and Thymol of Nigella sativa Seed. Journal of Food Composition and Analysis. 19 (2), 167–175.
Badan Pusat Statistik (2013). Perdangangan Luar Negeri – Impor. Volume III. 2013. Jakarta (ID): Badan Pusat Statistik.
Barnabás, B., Jäger, K., Feher, A. (2008). The effect of drought and heat stress on reproductive processes in cereals. Plant Cell Environ. 31, 11–38.
Benkaci-Ali, F.A., Baliouamer, Meklati, B.Y., Chemat, F. (2007). Chemical composition of seed essential oil from Algerian Nigella sativa extracted by microwave and hydrodistillation. Flavour Frag. J. 22, 148-153.
Botnick, I., Xue, W., Bar, E., Ibdah, M., Schwart, A., Joel, D.M., Lev, E., Fait, A., and Lewinsohn, E. (2012). Distribution of primary and specialized metabolites in Nigella sativa seeds, a spice with vast traditional and historical uses. Molecules. (17), 10159-10177.
Diacono M., Rubino, P., and Montemurro, F. (2013). Precision nitrogen management of wheat; a review. Agron. Sustain. Dev., 33(1), 219-241.
El-Sharkawy, G.A. and Abdel-Razzak, H.S. (2010). Response of cabbage plants (Brassica oleraceae var. capitata L.) to fertilization with chicken manure, mineral nitrogen fertilizer and humic acid. Alex. Sci. Exch. J. 31, 416-432.
Hadad, G.M., Salam, R.A.A. (2012). High performance liquid chromatography quantification of principal antioxidants in black seed (Nigella sativa L.) phyopharma-ceuticals. Journal of AOAC Int. 95(4), 1043-1047.
Hadi, H.S., Ghanepasand, F., Noormohamadi, G., Darzi, M.T. (2014). Influence of Manure Application and Nitrogen Fixing Bacteria on Yield and Yield Components of black cumin (Nigella sativa L.). Int J Adv Biol Biom Res. 2 (3): 628-635.
Hamid, M.E. (2012). Effect of Nigella sativa L. (black seed) extract on some clinically significant aerobic actinomycetes. J. of Medicinal Plants Research. 6, 339-341.
Herlina, Aziz, S.A., Kurniawati, A,, Faridah, D.N. (2017). Pertumbuhan dan produksi Habbatussauda (Nigella sativa L.) di tiga ketinggian Indonesia. J. Agron. Indonesia. 45(3): 323 -330.
Hidayati,Y.A., Benito, Tb., Kurnani, A., Marlina, E.T., Harlia, E. (2011). Kualitas pupuk cair hasil pengolahan sapi potong menggunakan Saccharomyces cereviceae. Jurnal Ilmu Ternak. 11(2): 104-107.
Hua, J. (2009). From freezing to scorching, transcriptional responses to temperature variations in plants. Curr. Opin. Plant Biol. 12:568–573.
Khoulenjani, M.B., & Salamati, M.S. (2011). Morphological reaction and yield of Nigella sativa L. to Fe and Zn. African Journal of Agricultural Research. 7, 2359-2362.
Lewinsohn, E., Botnick, I., Xue, W., Bar, E., Ibdah, M., Schwartz, A., …, Fait, A. (2012). Distibution of primary and specialized metabolites in Nigella sativa seeds, a spice with vast traditional and historical uses. J Molecules. 17:10159-10177.
Malhatora, S.K. (2006). Nigella. In: K.V. Peter (Eds.). Handbook of hebrbs & spices. Vol. 2. pp. 206-214. Cambridge, England: Woodhead Publishing Ltd.
Prasetyo, B.H. & Suriadikarta, D.A. (2006). Karakteristik, potensi dan teknologi pengelolaan tanah Ultisol untuk pengembangan pertanian lahan kering di Indonesia. Jurnal Litbang Pertanian. 2006. 25(2), 39-46.
Rabbani, M.A., Ghafoor, A., Masood, M.S. (2011). NARC-Kalonji: An early maturing and high yielding and variety of Nigella sativa released for cultivation in Pakistan. Pak J. Bot. 43:191-195.
Raihan, H.S. (2000). Pemupukan NPK dan ameliorasi lahan kering sulfat masam berdasarkan nilai uji tanah untuk tanaman jagung. J. Ilmu pertanian. 9 (1), 20-28
Ridwan, T., Ghulamadi, M., Kurniawati, A. (2014). Laju pertumbuhan dan produksi jintan hitam (Nigella sativa L.) dengan aplikasi pupuk kandang sapi dan fosfat alam. J. Agron. Indonesia. 42(2), 158-165. 10.24831/jai.v42i2.8435
Sakata, T., and Higashitani, A. (2008). Male sterility accompanied with abnormal anther development in plants–genes and environ-mental stresses with special reference to high temperature injury. Int. J. Plant Dev. Biol. 2, 42–51.
Suastika, I.W., Sutriadi, M.T., Kasno, A. (2005). Pengaruh pupuk kandang dan fosfat alam terhadap produktivitas jagung di typic hapludox dan plintic kandiudults Kalimantan Selatan. Halaman 191-201 dalam Prosiding Seminar Nasional Sumber Daya Tanah, Iklim, dan Pupuk Buku II, Bogor.
Talafih, K.A., Haddad, N.I., Hatar, B.I., Kharallah, K. (2007). Effect of some agricultural practises on the productivity of black cummin (Nigella sativa) grown under rainfed semi-arid conditions Jodan. Journal of Agricultural Sciences. 3:385-397.
Thakur, P., Kumar, S., Malik, J.A., Berger, J.D., Nayyar, H. (2010). Cold stress effects on reproductive development in grain crops, an overview. Environ. Exp. Bot. 67:429–443.
Tuncturk, M.R., Tuncturk, V., Ciftci. (2012). The Effect of varying Nitrogen doses on yield and some yield components of black cumin (Nigella sativa L.). Advances in Environ-mental Biology. 6(2), 855-858.
Wahid A, Gelani S, Ashraf M, Foolad MR. 2007. Heat tolerance in plants: an overview. Env. Exp Bot. 61(3), 199-223.
Wajs, A., Bonikowski, R., Kalemba, D. (2008). Composition of essential oil from seeds of Nigella sativa L. cultivated in Poland. Flavour and Fragrance Journal. 23(2),126–132.
How to Cite
HERLINA, Herlina; ANDRIANI, Evi. Variation in yield components and bioactive contents of black cumin seeds (Nigella sativa L.) with application of animal manures in ultisol lowland of Bengkulu. Buletin Agroteknologi, [S.l.], v. 1, n. 1, p. 12-20, june 2020. ISSN 2722-7235. Available at: <>. Date accessed: 21 oct. 2020. doi: