Supplementation of Inulin in Feed on Abundance of Lactic Acid Bacteria, Growth Performance and Feed Utilization of Tilapia (Oreochromis niloticus)

Authors

  • Panji Krisna Yuga Pamungkas Mulawarman University
  • Agustina Agustina Faculty of Fisheries and Marine Science, Mulawarman University
  • Sulistyawati Mulawarman University

DOI:

https://doi.org/10.32663/ja.v22i1.4312

Keywords:

growth, inulin, lactic acid bacteria, Oreochromis niloticus, prebiotic

Abstract

Inulin is a type of prebiotic that has been studied to be useful in increasing the growth of several fish species. This research was carried out to evaluate the effect of inulin supplementation on the abundance of lactic acid bacteria in the intestine, growth performance and feed utilization including weight growth, specific growth rate, and feed conversion ratio of tilapia (Oreochromis niloticus) fingerlings. Ten tilapia fish with an average weight of 4.41 ± 0.52 g were reared in plastic tanks with a volume of 30 L for 30 days. During rearing, tilapia are given commercial feed with inulin supplementation as a treatment, namely: 0.0; 1.5; 3.0 and 6.0 g/kg feed, three times a day ad satiation. The data obtained in this study was tested statistically using the one-way ANOVA test and Tukey's advanced test. The results of the study showed that the inulin supplementation treatment of 1.5 g/kg feed provided the highest abundance of lactic acid bacteria at the end of the study, the highest weight growth, specific growth rate and lowest feed conversion ratio (P<0.05) compared to other treatments. The highest total gut bacteria of tilapia fry at the end of the study was shown at a treatment dose of 3.0 g inulin/kg feed. Based on this, inulin at a dose of 1.5 g/kg feed can be used as a prebiotic for tilapia seeds to support their growth and feed utilization.

Author Biographies

  • Panji Krisna Yuga Pamungkas, Mulawarman University

    Aquaculture Department

  • Agustina Agustina, Faculty of Fisheries and Marine Science, Mulawarman University

    Aquaculture Department 

  • Sulistyawati, Mulawarman University

    Aquaculture department

References

Akhter, N., Wu, B., Memon, A.M., & Mohsin M. (2015). Probiotics and prebiotics associated with aquaculture: A review. Fish & Shellfish Immunology, 45, 733-741. DOI: https://doi.org/10.1016/j.fsi.2015.05.038

Akrami, R., Iri, Y., Rostami, H.K., & Mansour, M.R. (2013). Effect of dietary supplementation of fructooligosaccharide (FOS) on growth performance, survival, Lactobacillus bacterial population and hemato-immunological parameters of stellate sturgeon (Acipenser stellatus) juvenile. Fish & Shellfish Immunology, 35, 1235-1239. DOI: https://doi.org/10.1016/j.fsi.2013.07.039

Ali, S.S.R., Ambasankar, K., Nandakumar, S., Praveena, P.E., & Syamadaya, J. (2016). Effect of dietary prebiotic inulin on growth, body composition and gut microbiota of Asian seabass (Lates calcarifer). Animal Feed Science and Technology, 217, 87-94. DOI: https://doi.org/10.1016/j.anifeedsci.2016.04.011

Ali, S.S.R., Ambasankar, K., Praveena, P.E., Nandakumar, S., & Musthafa, M.S. (2018). Effect of dietary prebiotic inulin on histology, immuno-haematological and biochemical parameters of Asian seabass (Lates calcarifer). Aquaculture Research, 49, 2732-2740. DOI: https://doi.org/10.1111/are.13734

Begum, J. (2023). Health benefits of Tilapia. https://www.webmd.com/diet/health-benefits-tilapia [diakses 23 September 2023].

Boonanuntanasarn, S., Tiengtam, N., Pitaksong, T., Piromyou, P., & Teaumroong, N. (2017). Effects of dietary inulin and Jerusalem artichoke (Helianthus tuberosus) on intestinal microbiota community and morphology of Nile tilapia (Oreochromis niloticus) fingerlings. Aquaculture Nutrition, 1-11. DOI: https://doi.org/10.1111/anu.12600

Cui, X., Zhang, Q., Zhang, Q., Zhang, Y., Chen, H., Liu, G., & Zhu L. (2022). Research progress of the gut microbiome in hybrid fish. Microorganisms, 10(891), 1-21. DOI: https://doi.org/10.3390/microorganisms10050891

Dawood, M.A.O., & Koshio, S. (2016). Recent advances in the role of probiotics and prebiotics in carp aquaculture: A review. Aquaculture, 454, 243-251. DOI: http://dx.doi.org/10.1016/j.aquaculture.2015.12.033

Eshaghzadeh, H., Hoseinifar, S.H., Vahabzadeh, H., & Ringø, E. (2014). The effect of dietary inulin on growth performances, survival and digestive enzyme activities of common carp (Cyprinus carpio) fry. Aquaculture Nutrition, 1-6. DOI: https://doi.org/10.1111/anu.12155

Ghafarifarsani, H., Rashidian, G., Bagheri, T., Hoseinifar, S.H., & Van Doan, H. (2021). Study on growth enhancement and the protective effects of dietary prebiotic inulin on immunity responses of rainbow trout (Oncorhynchus mykiss) fry infected with Aeromonas hydrophila. Annals of Animal Science, 21(2), 543-559. DOI: http://dx.doi.org/10.2478/aoas-2020-0074

Hoseinifar, S.H., Mirvaghefi, A., Amiri B.M., Rostami, H.K., & Merrifield, D.L. (2011). The effects of oligofructose on growth performance, survival and autochthonous intestinal microbiota of beluga (Huso huso) juveniles. Aquaculture Nutrition, 17, 498-504. DOI: https://doi.org/10.1111/j.1365-2095.2010.00828.x

Hunt, A.O., Çetinkaya, M., Yilmaz, F.O., Yildirim, M., Berköz, M., & Yalin, S. (2019). Effect of Dietary Supplementation of Inulin on Growth Performance, Digestion Enzyme Activities and Antioxidant Status of Rainbow Trout (Oncorhynchus mykiss). Turkish Journal of Agriculture-Food Science and Technology, 7(9), 1344-1353. DOI: https://doi.org/10.24925/turjaf.v7i9.1344-1353.2581

Ibrahem, M.D., Fathi, M., Mesalhy, S., Abd El-Aty, A.M. (2010). Effect of dietary supplementation of inulin and vitamin C on the growth, hematology, innate immunity, and resistance of Nile tilapia (Oreochromis niloticus). Fish & Shellfish Immunology, 29, 241-246. DOI: https://doi.org/10.1016/j.fsi.2010.03.004

Joshna, M., & Ahilan, K. (2023). Is fish gut microflora influenced by inulin? https://www.feedandadditive.com/is-fish-gut-microflora-influenced-by-inulin/#:~:text=Research%20on%20the%20effect%20of,general%20disease%20resistance%20of%20fish. [diakses 23 September 2023].

Lauriano, E.R., Pergolizzi, S., Capillo, G., Kuciel, M., Alesci, A., & Faggio, C. (2016). Immunohistochemical characterization of Toll-like receptor 2 in gut epithelial cells and macrophages of goldfish Carassius auratus fed with a high-cholesterol diet. Fish & Shellfish Immunology, 59. DOI: https://doi.org/10.1016/j.fsi.2016.11.003

Ringø, E., Dimitroglou, A., Hoseinifar, S.H., & Davies, S.J. (2014). Prebiotics in finfish: an update. In: Aquaculture Nutrition: Gut Health, Probiotics and Prebiotics (Ringø, E & Merrifield, D. eds), in press. Wiley-Blackwell, Oxford, UK.

Roberfroid, M. (2007). Prebiotics: the concept revisited. Journal of Nutrition, 137, 830S-837S. DOI: https://doi.org/10.1093/jn/137.3.830s

Saputri, R.A., Widyorini, N., & Purnomo, P.W. (2016). Identifikasi dan Kelimpahan Bakteri pada Jenis Karang Acropora sp. di Reef Flat Terumbu Karang Pulau Panjang Jepara. Indonesian Journal of Fisheries Science and Technology, 12(1), 35-39. DOI: https://doi.org/10.14710/ijfst.12.1.35-39

Setyawan, A.A., Sukanto, & Widyastuti, E. (2014). Populasi Bakteri Asam Laktat pada Budidaya Ikan Nila yang Diberi Pakan Fermentasi Limbah Pertanian dengan Suplemen Enceng Gondok dan Probiotik. Scripta Biologica, 1(1), 91-95. DOI: https://doi.org/10.20884/1.sb.2014.1.1.32

Tiengtam, N., Khempaka, S., Paengkoum, P., & Boonanuntanasarn, S. (2015). Effects of inulin and Jerusalem artichoke (Helianthus tuberosus) as prebiotic ingredients in the diet of juvenile Nile tilapia (Oreochromis niloticus). Animal Feed Science and Technology, 207, 127-129. DOI: https://doi.org/10.1016/j.anifeedsci.2015.05.008

Wang, T., Zhang, N., Yu, X.B., Qiao, F., Chen, L.Q., Du, Z.Y., & Zhang, M.L. (2021). Inulin alleviates adverse metabolic syndrome and regulates intestinal microbiota composition in Nile tilapia (Oreochromis niloticus) fed with high-carbohydrate diet. The British Journal of Nutrition, 126(2), 161-171. DOI: https://doi.org/10.1017/S000711452000402X

Yones, A.M.A.S.M., Eissa, I.A.M., Ghobashy, M.A.E.A., & Marzok, S.S. (2019). Effects of dietary inulin as prebiotic on growth performance, immuno-haematological indices and ectoparasitic infection of fingerlings Nile tilapia, Oreochromis niloticus. Egyptian Journal of Histology, 43(1), 88-103. DOI: http://dx.doi.org/10.21608/ejh.2019.15495.1152

Published

2024-06-28

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